南校区座落在Brighton镇，紧邻罗切斯特市南部。该校区包括有Laboratory for Laser Energetics, the Center for Optics Manufacturing, the Center for Optoelectronics and Imaging, and the now defunct Nuclear Structure Research Laboratory (NSRL). 研究生住宿区Whipple Park也在此区。
Rochester Area Properties
C.E.K. Mees Observatory
Memorial Art Gallery
Mount Hope Campus